Figure 1. Two‐color in vivo reporters for splicing and translation. (a,b) Two variants of the splicing reporter with the reading frames for GFP and RFP shown in pale green and pale red, respectively. The two spliced transcripts expressed from the minigenes are shown underneath their diagrams. The reading frame expressed from the each transcript is highlighted in a brighter color; (c) The reporter for translation.

Figure 2. Example data from a screening experiment. (a) Screening plate images immediately after the addition of the compounds (left) and at the end of the treatment (right); (b) Median column intensities, excluding the wells belonging to the first and last rows, before (blue squares) and after LOWESS smoothing (red diamonds); (c) An example of the final outcome of the cell‐based screening assay. The regression slopes of the RFP: GFP ratio over the course of the treatment are plotted against the compound index. In this case, active compounds should have negative regression slopes indicating a decrease of the RFP: GFP ratio; (d) Treatment with decreasing doses of two compounds (8‐azaguanine and digoxin) of cells expressing the splicing (left) and translation (right) reporters. The wells in the first column of each image are incubated with DMSO; (e) RT‐PCR analysis of splicing after treatment with DMSO (left) and an active compound (right). Each panel shows the PCR products resolved either on denaturing polyacrylamide gel (image) or by capillary electrophoresis (chromatogram). The positions of the exon‐included and exon‐skipped isoforms are indicated by “ + ” and “ − ” signs, respectively.