Wiley-VCH - Proteomics in Practice

Westermeier, Reiner / Naven, Tom

Proteomics in Practice

A Guide to Successful Experimental Design

2., completely revised Edition - January 2008
2008, XVI, 384 Pages, Hardcover
ISBN 978-3-527-31941-1 - Wiley-VCH, Weinheim

Short description

Still the only concise practical guide, this edition combines the best features of a laboratory manual with a concise reference for successful experimental design. Full coverage of electrophoresis, liquid chromatography, mass spectrometry and further relevant techniques.

From the contents

Preface
Foreword
Abbreviations
Glossary of terms
INTRODUCTION
History
Critical points
Hypothesis driven approach vs. High Throughput
Complementarities of workflows
Concept of experimental planning
Success stories, highlights of useful references

PART I: PROTEOMICS TECHNOLOGY
Electrophoresis
Liquid Chromatography
Mass spectrometry
Bioinformatics tools / data mining
Methods under development

PART II. PRACTICAL MANUAL OF PROTEOME ANALYSIS
Strategy where to start
Gel-based workflow
LC-based workflow
Latest trends
Validation with Western blotting
Statistic evaluation

PART III: TROUBLE SHOOTING
Gel-based workflow
LC based workflow
References
Index

Principle of 2-D Electrophoresis - (Animation)
MALDI - ToF Mass Spectrometer - (Animation)
Reflectron - (Animation)
Post Source Decay Analysis with Reflectron - (Animation)
Electrospray Ionization - (Animation)

Animations of Proteomic Separations (Basic Principles)
Please note: The graphics of the animations are adjusted to a screen resolution of 1280 x 1024 pixels. This resolution is needed in order to display the full size of the graphics on your computer screen.