Figure 1. WaterLOGSY (a), STD (b), and CPMG (c) NMR experiments to identify specific interaction of a small‐molecule ligand to a protein.

Figure 2. STD competition screening experiments monitoring the methyl signal of a “spy” molecule to identify and rank fragment binders in screening.

Figure 3. ILOE peaks (indicated with red arrows) and their displacement attempts for the (a) hydrophobic and (b) more water‐soluble ILOE probe. Only specific ILOE peaks yield information on the relative binding mode (lower right). PtS, pantothenate synthetase.