RNA, RNA interference, ribozyme switches, microRNA, structural studies, aptamers, and more ...
The importance of RNA interference was recognized by the Nobel Assembly, who awarded the 2006 Nobel Prize in Physiology or Medicine jointly to Andrew Z. Fire (Stanford School of Medicine, USA) and Craig C. Mello (University of Massachusetts, USA) for their discovery of RNA interference—gene silencing by double-stranded RNA.
Thomas Tuschl, winner of the Wiley Prize in Biomedical Sciences in 2003, discussed RNA Interference and Small Interfering RNAs in a Minireview in ChemBioChem, 2001, 2, 239.
Transcriptome labelling: We describe a metabolic labelling approach for rapid generation of 13C bio-isotopologues of the C. elegans transcriptome to study its modifications by liquid-chromatography tandem high-resolution mass spectrometry (LC-HRMS). We identify the most abundant RNA modifications, study the response to environmental stress and demonstrate their dynamic behaviour.
Pieter van Delft, Alper Akay, Sabrina M. Huber, Christoph Bueschl, Konrad L. M. Rudolph, Tomás Di Domenico, Rainer Schuhmacher, Eric A. Miska, Shankar Balasubramanian
ChemBioChem, April 27, 2017, https://doi.org/10.1002/cbic.201700093 Read article
Seeing into the complex: The stoichiometry of the binding, thermal denaturation of complexes, and transition between triple-helical peptide nucleic acid (PNA)/microRNA and (PNA)2/microRNA complexes were characterized by 19F NMR spectroscopy (see figure).
Ville Tähtinen, Lotta Granqvist, Merita Murtola, Roger Strömberg, Pasi Virta
Chem. Eur. J., April 25, 2017, https://doi.org/10.1002/chem.201700601 Read article
T-cell immunity elicited by mRNA vaccines is heavily influenced by the delivery system. Cell penetrating peptide containing amphipathic RALA motifs condenses mRNA into nanocomplexes that efficiently translocate their mRNA cargo from endocytic vesicles to the cytosol. Through unique immune activation and enhanced expression, nucleoside modified mRNA complexed with RALA peptide induces potent antigen specific cytolytic T cells in vivo.
Vimal K. Udhayakumar, Ans De Beuckelaer, Joanne McCaffrey, Cian M. McCrudden, Jonathan L. Kirschman, Daryll Vanover, Lien Van Hoecke, Kenny Roose, Kim Deswarte, Bruno G. De Geest, Stefan Lienenklaus, Philip J. Santangelo, Johan Grooten, Helen O. McCarthy, Stefaan De Koker
Adv. Healthcare Mater., April 24, 2017, https://doi.org/10.1002/adhm.201601412 Read article
Indestructible: Structural investigation by CD spectroscopy, UV melting, NMR, and PAGE was performed on a highly symmetrical RNA G-quadruplex consisting of five tetrad layers which cannot be destroyed by common denaturing conditions. However, the cationic porphyrin TMPyP4 was shown to destabilize the complex.
Katharina Kulikov, Senada Nozinovic, Stephanie Kath-Schorr
ChemBioChem, April 11, 2017, https://doi.org/10.1002/cbic.201700028 Read article
Time-gated photoluminescence detection of a single terbium-donor-based Förster resonance energy transfer (FRET) pair to selectively quantify low nanomolar concentrations of multiple DNAs or microRNAs from a single sample with a single measurement is shown. Both molecular (Tb-to-dye) and nanoparticle (Tb-to-quantum-dot) FRET biosensing are demonstrated.
Xue Qiu, Jiajia Guo, Zongwen Jin, Igor L. Medintz, Niko Hildebrandt
Small, April 03, 2017, https://doi.org/10.1002/smll.201700332 Read article
Releasing siRNA: Binding and release of short interfering ribonucleic acid (siRNA) from lyotropic liquid crystalline lipid nanoparticles (LNPs) on a self-assembled monolayer was demonstrated by using the aid of a quartz crystal microbalance. Small-angle X-ray scattering (SAXS) was used to characterise the attached LNPs.
Behnoosh Tajik-Ahmadabad, Adam Mechler, Benjamin W. Muir, Keith McLean, Tracey M. Hinton, Frances Separovic, Anastasios Polyzos
ChemBioChem, April 03, 2017, https://doi.org/10.1002/cbic.201600613 Read article
How to get many products from a few instructors: We investigated the RNA-templated transfer reaction between two peptide–PNA conjugates based on native chemical ligation. Thioester reactivity, template architecture, affinity, but not peptide length are key to high-performance peptidyl transfer on RNA.
Margherita Di Pisa, Anett Hauser, Oliver Seitz
ChemBioChem, February 24, 2017, https://doi.org/10.1002/cbic.201600687 Read article
Mol. Inf., December 21, 2016, https://doi.org/10.1002/minf.201600132 Read article