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RNA, RNA interference, ribozyme switches, microRNA, structural studies, aptamers, and more ...

The importance of RNA interference was recognized by the Nobel Assembly, who awarded the 2006 Nobel Prize in Physiology or Medicine jointly to Andrew Z. Fire (Stanford School of Medicine, USA) and Craig C. Mello (University of Massachusetts, USA) for their discovery of RNA interference—gene silencing by double-stranded RNA.

Thomas Tuschl, winner of the Wiley Prize in Biomedical Sciences in 2003, discussed RNA Interference and Small Interfering RNAs in a Minireview in ChemBioChem, 2001, 2, 239.

Find all articles on RNA or just on RNA interference in Wiley Online Library...

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Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

Quantitative Electrochemical DNA Microarray on a Monolith Electrode with Ten Attomolar Sensitivity, 100 % Specificity, and Zero Background

Chip off the block: A DNA chip technology is developed to measure binding to a microarray on a monolith electrode by probing the local redox reaction on each spot. The redox peak, Rmax, was zero for nonbinding (ssDNA) and nonspecific binding. The binding is enhanced electrochemically in <30 mins to attain a 10−2 to 103 femtomolar dynamic range. The method is applied to measure microRNA extracted from plasma with no PCR or cDNA conversion.

[Communication]
Shobana Raghunath, Abhijeet Prasad, Rahul Tevatia, Jillian.R. Gunther, Santanu Roy, Sunil Krishnan, Ravi F. Saraf
ChemElectroChem, December 14, 2017, https://doi.org/10.1002/celc.201700983 Read article

Live-Cell MicroRNA Imaging through MnO2 Nanosheet-Mediated DD-A Hybridization Chain Reaction

Live‐Cell MicroRNA Imaging through MnO2 Nanosheet‐Mediated DD‐A Hybridization Chain Reaction

Nanosheets: small but mighty! We report an approach for live-cell miRNA imaging through a biodegradable MnO2 nanosheet-mediated donor–donor–acceptor (DD-A) FRET hybridization chain reaction. The target miR-21 triggered cascaded assembly of the liberated hairpin probes into long dsDNA polymers, which brought each the FAMs (donors) and TAMRA (acceptor) into close proximity to generate enhanced DD-A FRET signals.

[Communication]
Min Ou, Jin Huang, Xiaohai Yang, Xiaoxiao He, Ke Quan, Yanjing Yang, Nuli Xie, Jing Li, Kemin Wang
ChemBioChem, December 14, 2017, https://doi.org/10.1002/cbic.201700573 Read article

A T7 RNA Polymerase Mutant Enhances the Yield of 5′-Thienoguanosine-Initiated RNAs

A T7 RNA Polymerase Mutant Enhances the Yield of 5′‐Thienoguanosine‐Initiated RNAs

Improved 5′-modification of RNA: Premature termination of RNAs initiated with guanosine analogues has rendered it difficult to introduce 5′-guanosine modifications into RNAs generated by in vitro transcription with T7 RNA polymerase (T7RNAP). Here, we provide a solution by using a mutant T7RNAP with a decreased propensity for abortive transcription.

[Communication]
Seth Lyon, Venkat Gopalan
ChemBioChem, December 07, 2017, https://doi.org/10.1002/cbic.201700538 Read article

Precise Assembly of Synthetic Carriers of siRNA through a Series of Interlocked Thermodynamically Self-Regulated Processes

Precise Assembly of Synthetic Carriers of siRNA through a Series of Interlocked Thermodynamically Self‐Regulated Processes

This study introduces a synthetic carrier for siRNA delivery with customizable size, pH-responsive degradability, and flexibility to immobilize selected cell-targeting agents constructed precisely through a series of interlocked thermodynamically self-regulated processes. This system can accomplish all consecutive tasks, such as siRNA encapsulating, target cell recognizing, endosome escaping, siRNA releasing, and self-metabolizing, and be simple in structure and easy to formulate.

[Full Paper]
Jia Feng, Shun Chen, Xuemei Ge, Fei Wu, Guang Bai, Tuo Jin
Adv. Funct. Mater., November 30, 2017, https://doi.org/10.1002/adfm.201703207 Read article

Rewarming the Primordial Soup: Revisitations and Rediscoveries in Prebiotic Chemistry

Rewarming the Primordial Soup: Revisitations and Rediscoveries in Prebiotic Chemistry

A short history of primordial soup: In this essay we try to disclose some of the historical connections between the studies that have contributed to our current understanding of the emergence of catalytic RNA molecules and their components from an inanimate matter.

[Essay]
Raffaele Saladino, Judit E. Šponer, Jiří Šponer, Ernesto Di Mauro
ChemBioChem, November 22, 2017, https://doi.org/10.1002/cbic.201700534 Read article

Micro-scale RNA Interference using Iron Oxide Nanoparticle-modified Lentivirus

Micro‐scale RNA Interference using Iron Oxide Nanoparticle‐modified Lentivirus

A magnetic lentivirus has been used to achieve micro-scale RNA interference (RNAi) through EGFR silencing for localized micro-scale RNAi virotherapy in a NSCLC cell in vitro model.

[Full Paper]
Zi-Xian Liao, Ivan M. Kempson, Man-Jing Chen, Yu-Chen Fa, Kuo-Yen Huang, Li-Fang Wang
ChemNanoMat, October 25, 2017, https://doi.org/10.1002/cnma.201700251 Read article

Scaffold-Based microRNA Therapies in Regenerative Medicine and Cancer

Scaffold‐Based microRNA Therapies in Regenerative Medicine and Cancer

MicroRNA-based therapies hold immense potential to finely balance gene expression and reestablish tissue health, but their progress largely relies on the development of safe and effective delivery systems. In this Progress Report future opportunities and current challenges that exist in the emerging application of biomaterial scaffolds as microRNA delivery platforms for regenerative medicine and cancer treatments are illustrated.

[Progress Report]
Caroline M. Curtin, Irene Mencía Castaño, Fergal J. O'Brien
Adv. Healthcare Mater., October 25, 2017, https://doi.org/10.1002/adhm.201700695 Read article

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