CHAPTER 16 Ten Tips and Tricks to Handle and Clean Your Microscope 99
immersion objectives also require specially selected coverslips of a thickness of
0.17 mm, if the best image is required. Immersion objectives should only be used
in conjunction with a suitable, bubble-free immersion liquid.
Five Goodies for Optimum Results
Optimum results in microscopy depend not only on the cleanness of the microscope
optics, but also on perfect specimen preparation:
✔ Thickness of the specimen
✔ Staining method and intensity
✔ Refractive index and dispersion of the embedding or immersion medium
✔ In high-resolution microscopy: close distance of a living cell from the
cover slip
✔ Correct coverslip thickness (for example 0.17 ± 0.01 mm) to avoid spherical
aberration
