Brief Contents
About the Authors . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Introduction . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Part I: Basics of Microscopy. . . . . . . . . . . . . . . . . . . . . . . . . 15
Chapter 1: The Microscope and How It Works . . . . . . . . . . . . . . . . . 17
Chapter 2: What Microscopes Do – Physically. . . . . . . . . . . . . . . . . . 23
Chapter 3: Different Types of Microscopes. . . . . . . . . . . . . . . . . . . . 27
Part II: A Deeper Look into a Light Microscope. . 31
Chapter 4: Components of Light Microscopes . . . . . . . . . . . . . . . . . 33
Chapter 5: Types of Objectives . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 35
Chapter 6: Illumination. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 39
Chapter 7: Contrasting Techniques. . . . . . . . . . . . . . . . . . . . . . . . . . . 45
Part III: Sample Preparation in Light
Microscopy. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 49
Chapter 8: Sample Preparation Techniques and Equipment. . . . . 51
Chapter 9: Sample Preparation of Histological Tissue. . . . . . . . . . . 55
Chapter 10: Important Staining Techniques . . . . . . . . . . . . . . . . . . . . 57
Part IV: Digital Documentation. . . . . . . . . . . . . . . . . . . . . 59
Chapter 11: Digital Microscope Cameras. . . . . . . . . . . . . . . . . . . . . . . 61
Chapter 12: Microscope Software. . . . . . . . . . . . . . . . . . . . . . . . . . . . . 75
Chapter 13: A Brief Look Back. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 77
Part V: The Part of Tens. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 81
Chapter 14: Ten Basic Experiments . . . . . . . . . . . . . . . . . . . . . . . . . . . 83
Chapter 15: Ten Steps to Set up Köhler Illumination. . . . . . . . . . . . . 93
Chapter 16: Ten Tips and Tricks to Handle and Clean
Your Microscope. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 95