IN THIS CHAPTER
Histological slides
Fix slides
About cutting slices
The importance of staining in histology
Chapter 9
Sample Preparation
of Histological Tissue
Histology is the study of cells and tissues, which is typically done with a
transmitted light microscope. The preparation of histological samples
can vary greatly based on the inherent properties of the samples such as
size and hardness as well as expected post-processing, which includes planned
staining techniques or other down-stream applications. Samples for histological
observation may be obtained from different sources. These are, for example, large
samples, whole kidneys or tiny pieces of a gut. Typical samples for histology are:
✔✔extracted organs or parts of organs after biopsy
✔✔incisional biopsy tissue
✔✔punch biopsy pieces, which are normally used to examine skin
✔✔shave biopsy, also used for skin
✔✔curettings, normally taken from uterus or cervix
✔✔core biopsy extracted with a needle
Fix Histological Samples to Keep Them
Fit for Purpose
To prevent degeneration or drying-out, the specimen should be fixed as soon as
possible after the biopsy. For this primary fixation you put the fresh sample in a